NSAIDs acutely inhibit TRPC channels in freshly isolated rat glomeruli. Biochem Biophys Res Commun 2011 May 06;408(2):242-7
Date
04/09/2011Pubmed ID
21473850Pubmed Central ID
PMC3093766DOI
10.1016/j.bbrc.2011.04.005Scopus ID
2-s2.0-79955662243 (requires institutional sign-in at Scopus site) 21 CitationsAbstract
Using a novel approach for analysis of TRPC channel activity, we report here that NSAIDs are involved into regulation of TRPC channels in the podocytes of the freshly isolated decapsulated glomeruli. Fluorescence and electron microscopy techniques confirmed the integrity of podocytes in the glomeruli. Western blotting showed that TRPC1, 3 and 6 are highly expressed in the glomeruli. Single-channel patch clamp analysis revealed cation currents with distinct TRPC properties. This is the first report describing single TRPC-like currents in glomerular podocytes. Furthermore, our data provide a novel mechanism of NSAIDs regulation of TRPC channels, which might be implicated in maintaining the glomerular filtration barrier.
Author List
Ilatovskaya DV, Levchenko V, Ryan RP, Cowley AW Jr, Staruschenko AAuthor
Allen W. Cowley Jr PhD Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAnti-Inflammatory Agents, Non-Steroidal
Diclofenac
Glomerulosclerosis, Focal Segmental
Ibuprofen
Kidney Glomerulus
Male
Rats
Rats, Sprague-Dawley
TRPC Cation Channels
