Faculty Collaboration Database

Regulation of glucocorticoid receptors and Na-K ATPase activity by hydrocortisone in proximal tubular epithelial cells. In Vitro Cell Dev Biol 1988 Aug;24(8):811-6

Date

08/01/1988

Pubmed ID

2900828

DOI

10.1007/BF02623652

Scopus ID

2-s2.0-0023780185 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

The effect of hydrocortisone (HC) in modulating glucocorticoid receptors (GR) and sodium-potassium adenosine triphosphatase (Na-K ATPase) activity was studied in primary cultures of immunoisolated murine proximal tubular epithelial cells (PTEC). Utilizing monoclonal antibody against stage-specific embryonic antigen-1, a homogeneous population of PTEC was obtained in high yield. The cells were cultured to confluence and further treated for 48 h in serum-free growth medium containing no HC (control); 50 nM HC; or 50 nM HC plus 20 nM of the antiglucocorticoid, RU 38486. PTEC treated with 50 nM HC had 56% of GR binding and 160% Na-K ATPase activity as compared to controls (P less than 0.01). GR binding was abolished by incubation in RU 38486 whereas Na-K ATPase fell below control values (P less than 0.05). Brief incubations of HC-treated PTEC with 0.5 mM ouabain resulted in a fall in GR binding without a change in Na-K ATPase activity. These data indicate that in PTEC, HC regulates GR binding and they suggest that stimulation of Na-K ATPase activity is a direct biological response to this receptor-hormone interaction. Thus, primary cultures of immunoaffinity-isolated PTEC offer a good model system for investigating the molecular basis underlying the regulation of GR binding and postreceptor events influenced by glucocorticoids.

Author List

Ellis D, Sothi TD, Curthoys NP, Ballou B, Avner ED

Author

Ellis D. Avner MD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Antibodies, Monoclonal
Cell Separation
Cells, Cultured
Estrenes
Hexokinase
Hydrocortisone
Kidney Tubules, Proximal
Mice
Mifepristone
Ouabain
Receptors, Glucocorticoid
Sodium-Potassium-Exchanging ATPase
Time Factors
gamma-Glutamyltransferase