Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex. Methods Mol Biol 2016;1408:227-41
Date
03/12/2016Pubmed ID
26965126DOI
10.1007/978-1-4939-3512-3_15Scopus ID
2-s2.0-84961285244 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
Optogenetics is rapidly gaining acceptance as a preferred method to study specific neuronal cell types using light. Optogenetic neuromodulation requires the introduction of a cell-specific viral vector encoding for a light activating ion channel or ion pump and the utilization of a system to deliver light stimulation to brain. Here, we describe a two-part methodology starting with a procedure to inject an optogenetic AAV virus into rat cortex followed by a second procedure to surgically implant an optical cannula for light delivery to the deeper cortical layers.
Author List
Pawela C, DeYoe E, Pashaie RAuthor
Christopher Pawela PhD Associate Professor in the Biomedical Engineering department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCerebellar Cortex
Equipment Design
Gene Transfer Techniques
Genetic Vectors
Ion Channels
Ion Pumps
Light
Optogenetics
Photic Stimulation
Rats
Rhodopsin
Viruses
