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Gene-based neurotransmitter modulation in cerebellar granule neurons. J Neurochem 1997 Jan;68(1):204-12

Date

01/01/1997

Pubmed ID

8978727

DOI

10.1046/j.1471-4159.1997.68010204.x

Abstract

The human glutamic acid decarboxylase (GAD) gene was transferred into rat cerebellar granule neurons. Following adenoviral-mediated gene transfer, nearly 100% of the neurons had transgene expression that persisted for the duration of their survival in culture. GABA levels were elevated both in the growth media and in lysates of GAD-modified granule neurons. In GAD-modified neurons, extracellular GABA levels steadily increased with time, whereas intracellular GABA levels peaked 10 days after gene transfer. GAD-modified neurons released both glutamate and GABA into the surrounding media before and after potassium-induced stimulation, but only the release of glutamate was sensitive to potassium stimulation. These data suggest that glutamatergic neurons, which initially contained no detectable GABA, can be genetically modified to release GABA constitutively.

Author List

Fritz JD, Utz AL, Hale NM, Wu L, Powers AC, Verdoorn TA, Robertson D

Author

Jeffery Duane Fritz PhD Associate Professor in the Medical School Regional Campuses department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adenoviridae
Animals
Cells, Cultured
Cerebellum
Gene Transfer Techniques
Glutamate Decarboxylase
Glutamic Acid
Humans
Neurons
Neurotransmitter Agents
Rats
Rats, Sprague-Dawley
gamma-Aminobutyric Acid