Characterization of a partial cDNA clone encoding porcine skeletal muscle titin: comparison with rabbit and mouse skeletal muscle titin sequences. Comp Biochem Physiol B 1993 Jun;105(2):357-60
Date
06/01/1993Pubmed ID
8359022DOI
10.1016/0305-0491(93)90241-vAbstract
1. A cDNA fragment encoding 571 amino acid residues of porcine skeletal muscle titin was isolated from total RNA using RNA-PCR. 2. The porcine titin clone hybridized to a large RNA species (> 23 kb) in rabbit cardiac muscle, rabbit skeletal muscle and porcine skeletal muscle. 3. The porcine skeletal muscle titin clone encoded two types of 100-residue motifs and its amino acid sequence was 96% and 93% identical to the corresponding sequence of rabbit and mouse skeletal muscle titin, respectively. These results suggest that the titin sequence is highly conserved among mammalian species.
Author List
Fritz JD, Wolff JA, Greaser MLAuthor
Jeffery Duane Fritz PhD Associate Professor in the Medical School Regional Campuses department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Base Sequence
Blotting, Northern
Cloning, Molecular
Connectin
DNA
Membrane Proteins
Mice
Molecular Sequence Data
Muscle Proteins
Nucleic Acid Hybridization
Polymerase Chain Reaction
Protein Kinases
Rabbits
Swine
