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Cell alkalinization is not necessary and increased sodium influx is not sufficient for stimulated superoxide production. FEBS Lett 1986 Jul 28;203(2):267-72

Date

07/28/1986

Pubmed ID

3015676

DOI

10.1016/0014-5793(86)80756-6

Abstract

Preincubation of rabbit neutrophils for 5 min with the protein kinase C inhibitor H7 causes inhibition of the rise in intracellular pH but not the increase in Na+ influx or stimulated oxidative burst produced by the chemotactic factor formyl-methionyl-leucyl-phenylalanine. On the other hand, the stimulated superoxide production, but not the increase in Na+ influx produced by phorbol 12-myristate 13-acetate, is inhibited by H7. The effect is more pronounced on the rate than the extent of the stimulated superoxide release. Furthermore, cell acidification produced by the phorbol ester but not by the chemotactic factor is decreased in the presence of H7. These results suggest that most of the stimulated Na+ influx is not coupled to H+ efflux, in the case of the chemoattractant, the rise in intracellular pH is not necessary for stimulated superoxide production, the increase in Na+ influx, in the case of the phorbol ester, is not sufficient for the stimulation of the oxidative burst, and the sources of the H+ responsible for the stimulated pH drop are the various metabolic activities of the cell, including NADPH oxidation and activation of the hexose monophosphate shunt.

Author List

Molski TF, Ford C, Weisman SJ, Sha'afi RI

Author

Steven J. Weisman MD Professor in the Anesthesiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Animals
Hydrogen-Ion Concentration
Isoquinolines
N-Formylmethionine Leucyl-Phenylalanine
Neutrophils
Piperazines
Protein Kinase C
Rabbits
Sodium
Superoxides
Tetradecanoylphorbol Acetate
jenkins-FCD Prod-484 8aa07fc50b7f6d102f3dda2f4c7056ff84294d1d