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CD36 Provides Host Protection Against Klebsiella pneumoniae Intrapulmonary Infection by Enhancing Lipopolysaccharide Responsiveness and Macrophage Phagocytosis. J Infect Dis 2016 Dec 15;214(12):1865-1875

Date

09/30/2016

Pubmed ID

27683817

Pubmed Central ID

PMC5142085

DOI

10.1093/infdis/jiw451

Scopus ID

2-s2.0-85016050287 (requires institutional sign-in at Scopus site)   27 Citations

Abstract

Klebsiella pneumoniae remains an important cause of intrapulmonary infection and invasive disease worldwide. K. pneumoniae can evade serum killing and phagocytosis primarily through the expression of a polysaccharide capsule, but its pathogenicity is also influenced by host factors. We examined whether CD36, a scavenger receptor that recognizes pathogen and modified self ligands, is a host determinant of K. pneumoniae pathogenicity. Despite differences in serum sensitivity and virulence of 3 distinct K. pneumoniae (hypermucoviscous K1, research K2, and carbapenemase-producing ST258) strains, the absence of CD36 significantly increased host susceptibility to acute intrapulmonary infection by K. pneumoniae, regardless of strain. We demonstrate that CD36 enhances LPS responsiveness to K. pneumoniae to increase downstream cytokine production and macrophage phagocytosis that is independent of polysaccharide capsular antigen. Our study provides new insights into host determinants of K. pneumoniae pathogenicity and raises the possibility that functional mutations in CD36 may predispose individuals to K. pneumoniae syndromes.

Author List

Olonisakin TF, Li H, Xiong Z, Kochman EJ, Yu M, Qu Y, Hulver M, Kolls JK, St Croix C, Doi Y, Nguyen MH, Shanks RM, Mallampalli RK, Kagan VE, Ray A, Silverstein RL, Ray P, Lee JS

Author

Roy L. Silverstein MD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
CD36 Antigens
Female
Host-Pathogen Interactions
Klebsiella Infections
Klebsiella pneumoniae
Lipopolysaccharides
Macrophages
Male
Mice, Inbred C57BL
Mice, Knockout
Phagocytosis
Pneumonia, Bacterial