Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Effects of very small amounts of cholesterol on gel-phase phosphatidylcholine membranes. Biochim Biophys Acta 1986 Jan 29;854(2):318-20

Date

01/29/1986

Pubmed ID

3002471

DOI

10.1016/0005-2736(86)90125-2

Abstract

The mobility of 5-doxyl stearic acid spin label (5-SASL) in the gel phase of dipalmitoylphosphatidylcholine membranes between the main transition and subtransition temperatures was studied as a function of cholesterol content. Very small amounts of cholesterol (0.01-1 mol%) cause a dramatic increase in the mobility of 5-SASL. Temperature-drop experiments from 38 degrees C to 28 degrees C were made across the pretransition temperature and the rate of approach to equilibrium was measured. Cholesterol at low concentrations also affects this rate. The membrane reached equilibrium after 10 h in the absence of cholesterol, 3 h at 0.01 mol% cholesterol, and less than 10 min at 0.03 mol% cholesterol.

Author List

Subczynski WK, Kusumi A

Author

Witold K. Subczynski PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Cholesterol
Cyclic N-Oxides
Electron Spin Resonance Spectroscopy
Membrane Lipids
Phosphatidylcholines
Spin Labels
Temperature
Time Factors