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Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation. Stem Cell Res 2016 Nov;17(3):657-660

Date

12/10/2016

Pubmed ID

27934602

Pubmed Central ID

PMC5409870

DOI

10.1016/j.scr.2016.11.010

Scopus ID

2-s2.0-85028043697   3 Citations

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.

Author List

Afzal MZ, Gartz M, Klyachko EA, Khan SS, Shah SJ, Gupta S, Shapiro AD, Vaughan DE, Strande JL

Author

Melanie Gartz PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Base Sequence
Cell Differentiation
Cell Line
Cellular Reprogramming
DNA Mutational Analysis
Embryoid Bodies
Female
Genetic Vectors
Homozygote
Humans
Induced Pluripotent Stem Cells
Karyotype
Microscopy, Fluorescence
Mutagenesis, Insertional
Plasminogen Activator Inhibitor 1
Sendai virus
Transcription Factors
Urine