Detection of oxygen consumption during very early stages of lipid peroxidation by ESR nitroxide spin probe method. Biochim Biophys Acta 1985 Dec 05;821(2):259-63
Date
12/05/1985Pubmed ID
2998470DOI
10.1016/0005-2736(85)90095-1Scopus ID
2-s2.0-0022346137 (requires institutional sign-in at Scopus site) 10 CitationsAbstract
Oxygen consumption during the very early stages of the spontaneous peroxidation of egg yolk phosphatidylcholine membranes was studied by monitoring the oxygen concentration in the aqueous phase of the sample using a spin-probe closed-chamber method. The method depends on the broadening by oxygen of the proton superhyperfine lines of the electron spin resonance spectra of the nitroxide radical spin probe 3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl. It is concluded that this method is useful in monitoring lipid peroxidation and that it monitors the onset of the peroxidation process before the commonly used thiobarbituric acid assay detects the peroxidation products.
Author List
Subczynski WK, Kusumi AAuthor
Witold K. Subczynski PhD Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Cyclic N-OxidesElectron Spin Resonance Spectroscopy
Lipid Bilayers
Lipid Peroxides
Methods
Nitrogen Oxides
Oxygen Consumption
Phosphatidylcholines
Spin Labels
