Faculty Collaboration Database

Noninvasive monitoring of tissue hemoglobin using UV-VIS diffuse reflectance spectroscopy: a pilot study. Opt Express 2009 Dec 21;17(26):23396-409

Date

01/07/2010

Pubmed ID

20052047

DOI

10.1364/OE.17.023396

Scopus ID

2-s2.0-73949109208 (requires institutional sign-in at Scopus site)   42 Citations

Abstract

We conducted a pilot study on 10 patients undergoing general surgery to test the feasibility of diffuse reflectance spectroscopy in the visible wavelength range as a noninvasive monitoring tool for blood loss during surgery. Ratios of raw diffuse reflectance at wavelength pairs were tested as a first-pass for estimating hemoglobin concentration. Ratios can be calculated easily and rapidly with limited post-processing, and so this can be considered a near real-time monitoring device. We found the best hemoglobin correlations were when ratios at isosbestic points of oxy- and deoxyhemoglobin were used, specifically 529/500 nm. Baseline subtraction improved correlations, specifically at 520/509 nm. These results demonstrate proof-of-concept for the ability of this noninvasive device to monitor hemoglobin concentration changes due to surgical blood loss. The 529/500 nm ratio also appears to account for variations in probe pressure, as determined from measurements on two volunteers.

Author List

Bender JE, Shang AB, Moretti EW, Yu B, Richards LM, Ramanujam N

Author

Bing Yu PH.D. Assistant Professor of Biomedical Engineering in the Biomedical Engineering department at Marquette University

MESH terms used to index this publication - Major topics in bold

Biomarkers
Blood Chemical Analysis
Blood Loss, Surgical
Diagnosis, Computer-Assisted
Feasibility Studies
Hemoglobins
Humans
Oxygen
Pilot Projects
Postoperative Hemorrhage
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet