Faculty Collaboration Database

Renal Delivery of Anti-microRNA Oligonucleotides in Rats. Methods Mol Biol 2017;1527:409-419

Date

01/25/2017

Pubmed ID

28116733

DOI

10.1007/978-1-4939-6625-7_31

Scopus ID

2-s2.0-85011003449

Abstract

MicroRNAs are endogenous small, non-protein-coding RNA molecules that play an important role in the regulation of a wide variety of cellular functions and disease processes. A novel role for microRNAs in the development of hypertension and hypertensive tissue injury is emerging in recent studies. Development of hypertension involves multiple organ systems and cannot be modeled in vitro. Therefore, the ability to experimentally alter genes, gene products, or biological pathways, including microRNAs, in an organ-specific manner in intact animal models is particularly valuable to hypertension research. The kidney plays a central role in the long-term regulation of arterial blood pressure. In this chapter, we describe a detailed protocol for using a renal interstitial injection method to deliver anti-miR oligonucleotides to knock down microRNA specifically in the kidney in conscious rats.

Author List

Usa KS, Liu Y, Kurth T, Kriegel AJ, Mattson DL, Cowley AW Jr, Liang M

Authors

Allen W. Cowley Jr PhD Professor in the Physiology department at Medical College of Wisconsin
Alison J. Kriegel PhD Associate Professor in the Physiology department at Medical College of Wisconsin
Mingyu Liang PhD Center Director, Professor in the Physiology department at Medical College of Wisconsin
Yong Liu PhD Assistant Professor in the Physiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Hypertension
Kidney
MicroRNAs
Oligonucleotides
Rats