Faculty Collaboration Database

Changes of the inducible heat shock protein 70 mRNA level in rat retina after ischemia and reperfusion. Ophthalmic Res 1998;30(5):291-4

Date

08/15/1998

Pubmed ID

9704332

DOI

10.1159/000055487

Scopus ID

2-s2.0-0031851004 (requires institutional sign-in at Scopus site)   18 Citations

Abstract

The heat shock protein 70 (HSP-70) mRNA level was evaluated in Long Evans rat retinas after ischemia and after reperfusion following ischemia. Retinal ischemia was induced by ligation of the optic nerve and vessels. Rats were sacrificed after 90 min of ischemia or 120 min of reperfusion following ischemia. Retinas were dissected. Total mRNA was extracted and inducible HSP-70 (iHSP-70) gene expression was analyzed by quantification of transcripts using an RT-PCR assay. Results were expressed in arbitrary units as a ratio of the optical density of iHSP-70/beta-actin electrophoretic bands. iHSP-70 gene expression was 0.220 +/- 0.027 (n = 5), 0.502 +/- 0.045 (n = 5) and 0.468 +/- 0.032 (n = 5) for the sham-operated, ischemia only and ischemia and reperfusion groups, respectively. There was a statistically significant difference between the control and ischemia groups, and between the control and ischemia and reperfusion groups (p < 0.001), suggesting a rapid HSP-70 mRNA expression of the retina due to an ischemic injury.

Author List

Lewden O, Garcher C, Assem M, Morales C, Rochette L, Bron AM

Author

Mahfoud Assem PharmD Associate Professor in the School of Pharmacy Administration department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
DNA Primers
Gene Expression
HSP70 Heat-Shock Proteins
Male
RNA
RNA, Messenger
Rats
Reperfusion
Reperfusion Injury
Retina