Faculty Collaboration Database

Pattern of superoxide dismutase enzymatic activity and RNA changes in rat heart ventricles after myocardial infarction. Am J Pathol 1997 Aug;151(2):549-55

Date

08/01/1997

Pubmed ID

9250167

Pubmed Central ID

PMC1858014

Scopus ID

2-s2.0-0030793002 (requires institutional sign-in at Scopus site)   38 Citations

Abstract

A multiplex reverse transcription polymerase chain reaction assay was designed to measure manganese superoxide dismutase (MnSOD) and CuZnSOD mRNAs in the left and right ventricles of rat hearts after myocardial infarction induced by occlusion of the left coronary artery. These data were compared with changes in enzymatic activities. In the left ventricle, Mn-SOD RNA increased significantly at 6 hours, peaked at 12 hours (490 +/- 38 arbitrary units), and progressively decreased (127 +/- 21 arbitrary units at 48 hours). In contrast, there was a steady accumulation of transcripts in the right ventricle up to 48 hours. In both ventricles, the changes in the MnSOD mRNA and protein content were not associated with proportional variations in enzymatic activity. There was no characteristic alteration of the CuZnSOD system in either ventricle over the 48-hour period. These results demonstrate that infarction selectively activates the MnSOD gene in the viable myocardium of both ventricles. They suggest that MnSOD may be involved in the adaptive response of myocytes to the overloading stress.

Author List

Assem M, Teyssier JR, Benderitter M, Terrand J, Laubriet A, Javouhey A, David M, Rochette L

Author

Mahfoud Assem PharmD Associate Professor in the School of Pharmacy Administration department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Heart Ventricles
Male
Myocardial Infarction
Polymerase Chain Reaction
RNA, Messenger
Rats
Rats, Wistar
Superoxide Dismutase