Adaptation of bioassay to detect endothelium-derived relaxing factors from the canine atrial endocardium. Rev Bras Cir Cardiovasc 2009;24(2):225-32
Date
09/22/2009Pubmed ID
19768303DOI
10.1590/s0102-76382009000200020Scopus ID
2-s2.0-70350662563 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
OBJECTIVE: The aim of this study was to assess the release of endothelium-derived relaxing factors from the endocardium of canine atrial appendage.
METHODS: To study the release of endothelium-derived relaxing factor (EDRF) from intact atrial endocardial endothelium, tube-shaped sutures of canine atrial appendages were performed and effluents from these tubes were bioassayed (isolated perfused organ chamber system) for detection of EDRF in canine coronary artery.
RESULTS: Effluent from the right atrial appendage caused a relaxation of 58.4 +/- 10.1% and the left atrial appendage 74.9 +/- 8.5% from the initial prostagladin F2alpha contraction in coronary artery. No significant statistical difference was detected in effluent from the right and left atrial appendages. This relaxation was abolished by treating the heart tubes with Triton X-100 and reduced by treatment with LNMMA, a competitive inhibitor of nitric oxide and with indomethacin, an inhibitor of the cyclo-oxygenase pathway, also indicating the release of vasodilatory prostanoids from the endocardial endothelium.
CONCLUSION: This study showed for the first time, in vitro luminal release of EDRF and prostacyclin from the canine heart atrium. The ability of the endocardial endothelium to produce these factors could play an important role in preventing thrombus formation in the cardiac chambers.
Author List
Chua YL, Evora PR, Celotto AC, Pearson PJ, Discigil B, Schaff HVAuthor
Paul Joseph Pearson MD, PhD Chief, Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
6-Ketoprostaglandin F1 alphaAnalysis of Variance
Animals
Biological Assay
Coronary Vessels
Cyclooxygenase Inhibitors
Dogs
Endocardium
Endothelium-Dependent Relaxing Factors
Enzyme Inhibitors
Female
Heart Atria
Indomethacin
Male
Nitric Oxide
omega-N-Methylarginine
