Faculty Collaboration Database

High-frequency ultrasonic waves cause endothelial dysfunction on canine epicardial coronary arteries. Rev Bras Cir Cardiovasc 2008;23(2):190-6

Date

09/30/2008

Pubmed ID

18820781

DOI

10.1590/s0102-76382008000200007

Scopus ID

2-s2.0-53049089463 (requires institutional sign-in at Scopus site)   2 Citations

Abstract

OBJECTIVE: Application of ultrasound energy by an endarterectomy probe can facilitate the removal of atheromatous plaque, but the effect of this procedure on surrounding vessel structure and function is still a matter of experimental investigations.

METHODS: To determine whether ultrasound energy impairs the production of nitric oxide or damages vascular smooth muscle function, isolated canine epicardial coronary artery segments were exposed to either high (25 W) or low (0-10 W) ultrasonic energy outputs, for 15 seconds, using an endarterectomy device prototype. After exposure, segments of epicardial coronary artery were studied in organ chambers. The following drugs were used: adenosine diphosphate (ADP), acetylcholine (Ach) and sodium fluoride (NaF) to study endothelium-dependent relaxation and sodium nitroprusside (SNP) and isoproterenol to evaluate endothelium-independent relaxation.

RESULTS: Application of high ultrasonic energy power impaired endothelium-dependent relaxation to ADP (10(-9)-10(-4) M), Ach (10(-9)-10(-4) M) and NaF (0.5-9.5 mM) in epicardial coronary arteries. However, low ultrasound energy output at the tip of the probe did not alter the endothelium-dependent relaxation (either maximal relaxation or EC50) to the same agonists. Vascular smooth muscle relaxation to isoproterenol (10(-9)-10(-5) M) or SNP (10(-9)-10(-6) M) was unaltered following exposure to either low or high ultrasonic energy outputs.

CONCLUSION: These experiments currently prove that ultrasonic energy changes endothelial function of epicardial coronary arteries at high power. However, ultrasound does not alter the ability of vascular smooth muscle of canine epicardial coronary arteries to relax.

Author List

Discigil B, King RM, Pearson PJ, Capellini VK, Rodrigues AJ, Schaff HV, Evora PR

Author

Paul Joseph Pearson MD, PhD Chief, Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Acetylcholine
Adenosine Diphosphate
Analysis of Variance
Animals
Coronary Vessels
Dogs
Endarterectomy
Endothelium, Vascular
Female
Isoproterenol
Male
Models, Animal
Muscle, Smooth, Vascular
Nitric Oxide
Nitroprusside
Sodium Fluoride
Ultrasonic Therapy
Ultrasonography, Interventional
Vasodilation