Faculty Collaboration Database

Bioassay of EDRF from internal mammary arteries: implications for early and late bypass graft patency. Ann Thorac Surg 1992 Dec;54(6):1078-84

Date

12/01/1992

Pubmed ID

1449290

DOI

10.1016/0003-4975(92)90073-d

Scopus ID

2-s2.0-0026619476 (requires institutional sign-in at Scopus site)   75 Citations

Abstract

To study the basal, luminal release of endothelium-derived relaxing factor, 35-mm segments of canine internal mammary artery (IMA) were cannulated and perfused at 5 mL/min in vitro with physiological salt solution. Vasoactive properties of the effluent were bioassayed on coronary artery smooth muscle. Effluent from IMAs produced significant vasodilation of the bioassay ring compared with effluent from a prosthetic conduit (n = 24; p < 0.05). The vasodilation by the effluent could be eliminated by mechanically removing the intima of the IMA, or by treating the IMA segments with NG-monomethyl-L-arginine or NG-nitro-L-arginine, two competitive inhibitors of nitric oxide synthesis from L-arginine; vasodilation was not influenced by treatment with indomethacin. In 83% of the superfusion experiments, effluent from the left IMA induced greater relaxation of the bioassay ring than did effluent from the right IMA. In addition, the average vasodilation induced by left IMA effluent was 28% +/- 2.3% versus 17.4% +/- 3.1% for the right (n = 24; p < 0.05). However, in organ chamber experiments, right and left IMAs exhibited comparable endothelium-dependent vasodilation to acetylcholine (n = 6). Because endothelium-derived relaxing factor induces vasodilation and also inhibits platelet adhesion, platelet aggregation, and atherogenesis, luminal release of endothelium-derived relaxing factor by the IMA could contribute to superior results when the artery is used in bypass grafting.

Author List

Pearson PJ, Evora PR, Schaff HV

Author

Paul Joseph Pearson MD, PhD Chief, Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Acetylcholine
Animals
Biological Assay
Coronary Vessels
Dinoprost
Dogs
Evaluation Studies as Topic
In Vitro Techniques
Indomethacin
Mammary Arteries
Nitric Oxide
Nitroprusside
Norepinephrine
Vascular Patency
Vasodilation