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Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation. Stem Cell Res 2017 Jan;18:41-44

Date

04/12/2017

Pubmed ID

28395801

Pubmed Central ID

PMC5939958

DOI

10.1016/j.scr.2016.12.003

Scopus ID

2-s2.0-85028072477   6 Citations

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subjects heterozygous for a novel Plasminogen Activator Inhibitor-1 (PAI-1) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.

Author List

Afzal MZ, Gartz M, Klyachko EA, Khan SS, Shah SJ, Gupta S, Shapiro AD, Vaughan DE, Strande JL

Author

Melanie Gartz PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Cell Culture Techniques
Cell Differentiation
Cell Line
Cellular Reprogramming
Embryoid Bodies
Female
Genetic Vectors
Heterozygote
Humans
Induced Pluripotent Stem Cells
Karyotype
Microscopy, Fluorescence
Plasminogen Activator Inhibitor 1
Polymorphism, Genetic
Sendai virus
Urine