Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation. Stem Cell Res 2017 Jan;18:41-44
Date
04/12/2017Pubmed ID
28395801Pubmed Central ID
PMC5939958DOI
10.1016/j.scr.2016.12.003Scopus ID
2-s2.0-85028072477 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subjects heterozygous for a novel Plasminogen Activator Inhibitor-1 (PAI-1) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.
Author List
Afzal MZ, Gartz M, Klyachko EA, Khan SS, Shah SJ, Gupta S, Shapiro AD, Vaughan DE, Strande JLAuthor
Melanie Gartz PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Cell Culture TechniquesCell Differentiation
Cell Line
Cellular Reprogramming
Embryoid Bodies
Female
Genetic Vectors
Heterozygote
Humans
Induced Pluripotent Stem Cells
Karyotype
Microscopy, Fluorescence
Plasminogen Activator Inhibitor 1
Polymorphism, Genetic
Sendai virus
Urine