Faculty Collaboration Database

Generation of human iPSCs from urine derived cells of a non-affected control subject. Stem Cell Res 2017 Jan;18:33-36

Date

04/12/2017

Pubmed ID

28395799

Pubmed Central ID

PMC5389122

DOI

10.1016/j.scr.2016.12.008

Scopus ID

2-s2.0-85028089891 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.

Author List

Afzal MZ, Gartz M, Klyachko EA, Khan SS, Shah SJ, Gupta S, Shapiro AD, Vaughan DE, Strande JL

Author

Melanie Gartz PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Base Sequence
Cell Culture Techniques
Cell Line
Cellular Reprogramming
Embryoid Bodies
Female
Genetic Vectors
Genotype
Heterozygote
Homozygote
Humans
Induced Pluripotent Stem Cells
Karyotype
Microscopy, Fluorescence
Plasminogen Activator Inhibitor 1
Sendai virus
Sequence Analysis, DNA
Transcription Factors
Urine