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Single and combinatorial chromatin coupling events underlies the function of transcript factor Kr??ppel-like factor 11 in the regulation of gene networks. BMC Mol Biol 2014 May 25;15:10

Date

06/03/2014

Pubmed ID

24885560

Pubmed Central ID

PMC4049485

DOI

10.1186/1471-2199-15-10

Scopus ID

2-s2.0-84902078010   5 Citations

Abstract

BACKGROUND: Kr??ppel-like factors (KLFs) are a group of master regulators of gene expression conserved from flies to human. However, scant information is available on either the mechanisms or functional impact of the coupling of KLF proteins to chromatin remodeling machines, a deterministic step in transcriptional regulation.

RESULTS AND DISCUSSION: In the current study, we use genome-wide analyses of chromatin immunoprecipitation (ChIP-on-Chip) and Affymetrix-based expression profiling to gain insight into how KLF11, a human transcription factor involved in tumor suppression and metabolic diseases, works by coupling to three co-factor groups: the Sin3-histone deacetylase system, WD40-domain containing proteins, and the HP1-histone methyltransferase system. Our results reveal that KLF11 regulates distinct gene networks involved in metabolism and growth by using single or combinatorial coupling events.

CONCLUSION: This study, the first of its type for any KLF protein, reveals that interactions with multiple chromatin systems are required for the full gene regulatory function of these proteins.

Author List

Calvo E, Grzenda A, Lomberk G, Mathison A, Iovanna J, Urrutia R

Authors

Gwen Lomberk PhD Professor in the Surgery department at Medical College of Wisconsin
Angela Mathison PhD Assistant Professor in the Surgery department at Medical College of Wisconsin
Raul A. Urrutia MD Center Director, Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Apoptosis Regulatory Proteins
Cell Cycle Proteins
Cells, Cultured
Chromatin
Chromatin Assembly and Disassembly
Gene Expression Regulation
Gene Regulatory Networks
Genome-Wide Association Study
Histone-Lysine N-Methyltransferase
Humans
Repressor Proteins
Transcription Factors
Transcription, Genetic