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Low-density lipoprotein preparation by combined diafiltration and ultracentrifugation. Anal Biochem 1988 Oct;174(1):121-7

Date

10/01/1988

Pubmed ID

3218726

DOI

10.1016/0003-2697(88)90525-8

Scopus ID

2-s2.0-0023701117   5 Citations

Abstract

A method for isolating low-density lipoprotein by combining diafiltration and ultracentrifugation is described. Diafiltration separates plasma components by use of an ultrafiltration membrane that excludes particles of molecular weight greater than 300,000. The retentate is concentrated three- to fourfold by ultrafiltration, allowing large-scale preparation of low-density lipoprotein. Low-density lipoprotein prepared in this manner is similar in physical, chemical, and biologic properties to low-density lipoprotein isolated by sequential density ultracentrifugation alone. When low-density lipoprotein, prepared by either method, was added to human umbilical vein endothelial cell cultures, no cytotoxicity was observed. The techniques described reduce the demand on multiple rotors and ultracentrifuges for large-scale preparation of low-density lipoprotein suitable and often needed for tissue culture studies.

Author List

Pritchard KA Jr, Holland JA, Rogers NJ, Crean CC, Britton TE, Onigman P, Stemerman MB

Author

Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Arteriosclerosis
Blood Proteins
Cell Survival
Cells, Cultured
Endothelium, Vascular
Filtration
Humans
Lipoproteins, LDL
Molecular Weight
Ultracentrifugation
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a