Low-density lipoprotein preparation by combined diafiltration and ultracentrifugation. Anal Biochem 1988 Oct;174(1):121-7
Date
10/01/1988Pubmed ID
3218726DOI
10.1016/0003-2697(88)90525-8Scopus ID
2-s2.0-0023701117 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
A method for isolating low-density lipoprotein by combining diafiltration and ultracentrifugation is described. Diafiltration separates plasma components by use of an ultrafiltration membrane that excludes particles of molecular weight greater than 300,000. The retentate is concentrated three- to fourfold by ultrafiltration, allowing large-scale preparation of low-density lipoprotein. Low-density lipoprotein prepared in this manner is similar in physical, chemical, and biologic properties to low-density lipoprotein isolated by sequential density ultracentrifugation alone. When low-density lipoprotein, prepared by either method, was added to human umbilical vein endothelial cell cultures, no cytotoxicity was observed. The techniques described reduce the demand on multiple rotors and ultracentrifuges for large-scale preparation of low-density lipoprotein suitable and often needed for tissue culture studies.
Author List
Pritchard KA Jr, Holland JA, Rogers NJ, Crean CC, Britton TE, Onigman P, Stemerman MBAuthor
Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
ArteriosclerosisBlood Proteins
Cell Survival
Cells, Cultured
Endothelium, Vascular
Filtration
Humans
Lipoproteins, LDL
Molecular Weight
Ultracentrifugation
