Reliability and Repeatability of Cone Density Measurements in Patients With Stargardt Disease and RPGR-Associated Retinopathy. Invest Ophthalmol Vis Sci 2017 Jul 01;58(9):3608-3615
Date
07/25/2017Pubmed ID
28738413Pubmed Central ID
PMC5525557DOI
10.1167/iovs.17-21904Scopus ID
2-s2.0-85026292711 (requires institutional sign-in at Scopus site) 36 CitationsAbstract
PURPOSE: To assess reliability and repeatability of cone density measurements by using confocal and (nonconfocal) split-detector adaptive optics scanning light ophthalmoscopy (AOSLO) imaging. It will be determined whether cone density values are significantly different between modalities in Stargardt disease (STGD) and retinitis pigmentosa GTPase regulator (RPGR)-associated retinopathy.
METHODS: Twelve patients with STGD (aged 9-52 years) and eight with RPGR-associated retinopathy (aged 11-31 years) were imaged using both confocal and split-detector AOSLO simultaneously. Four graders manually identified cone locations in each image that were used to calculate local densities. Each imaging modality was evaluated independently. The data set consisted of 1584 assessments of 99 STGD images (each image in two modalities and four graders who graded each image twice) and 928 RPGR assessments of 58 images (each image in two modalities and four graders who graded each image twice).
RESULTS: For STGD assessments the reliability for confocal and split-detector AOSLO was 67.9% and 95.9%, respectively, and the repeatability was 71.2% and 97.3%, respectively. The differences in the measured cone density values between modalities were statistically significant for one grader. For RPGR assessments the reliability for confocal and split-detector AOSLO was 22.1% and 88.5%, respectively, and repeatability was 63.2% and 94.5%, respectively. The differences in cone density between modalities were statistically significant for all graders.
CONCLUSIONS: Split-detector AOSLO greatly improved the reliability and repeatability of cone density measurements in both disorders and will be valuable for natural history studies and clinical trials using AOSLO. However, it appears that these indices may be disease dependent, implying the need for similar investigations in other conditions.
Author List
Tanna P, Kasilian M, Strauss R, Tee J, Kalitzeos A, Tarima S, Visotcky A, Dubra A, Carroll J, Michaelides MAuthors
Joseph J. Carroll PhD Director, Professor in the Ophthalmology and Visual Sciences department at Medical College of WisconsinSergey S. Tarima PhD Associate Professor in the Institute for Health and Equity department at Medical College of Wisconsin
Alexis M. Visotcky Biostatistician III in the Institute for Health and Equity department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AdolescentAdult
Cell Count
Child
Eye Proteins
Female
Humans
Macular Degeneration
Male
Microscopy, Confocal
Middle Aged
Observer Variation
Ophthalmoscopy
Reproducibility of Results
Retinal Cone Photoreceptor Cells
Retinitis Pigmentosa
Tomography, Optical Coherence
