Faculty Collaboration Database

Galactosylation does not prevent the rapid clearance of long-term, 4 degrees C-stored platelets. Blood 2008 Mar 15;111(6):3249-56

Date

12/22/2007

Pubmed ID

18096766

DOI

10.1182/blood-2007-06-097295

Scopus ID

2-s2.0-42449087743 (requires institutional sign-in at Scopus site)   78 Citations

Abstract

Cold storage of platelets for transfusion is desirable to extend platelet storage times and to prevent bacterial growth. However, the rapid clearance of cold-stored platelets prevents their use. A novel method for preventing the rapid clearance of cold-stored platelets has previously been developed in a murine model. Cold storage induces the clustering and recognition of exposed beta-N-acetylglucosamine (betaGlcNAc) on platelet surfaces. Glycosylation of betaGlcNAc residues with uridine 5'-diphosphogalactose (UDP-galactose) results in the normal survival of short-term (2 h) 0 degrees C-stored murine platelets. Based on this finding, we developed a similar glycosylation process by adding UDP-galactose to human apheresis platelets. A phase 1 clinical trial was conducted transfusing radiolabeled autologous apheresis platelets stored for 48 hours at 4 degrees C with or without pretreatment with UDP-galactose. In contrast to the murine study, galactosylation of human platelets did not prevent the accelerated platelet clearance routinely observed after 4 degrees C storage. We next developed a murine model of platelet storage for 48 hours at 4 degrees C and showed that UDP-galactose treatment of murine platelets also did not prevent their rapid clearance, in agreement with the human platelet study. We conclude that different mechanisms of clearance may exist for short- and long-term cold-stored platelets.

Author List

Wandall HH, Hoffmeister KM, Sørensen AL, Rumjantseva V, Clausen H, Hartwig JH, Slichter SJ

Author

Karin Hoffmeister MD Professor in the Biochemistry department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Blood Component Removal
Blood Platelets
Blood Preservation
Cell Survival
Cold Temperature
Female
Galactose
Humans
Male
Mice
Mice, Inbred C57BL
Platelet Transfusion
Time Factors
Uridine Diphosphate Galactose