Faculty Collaboration Database

A rapid Western blotting protocol for the Xenopus oocyte. Cold Spring Harb Protoc 2013 Mar 01;2013(3)

Date

03/05/2013

Pubmed ID

23457341

Pubmed Central ID

PMC4017335

DOI

10.1101/pdb.prot072793

Scopus ID

2-s2.0-84874750701   11 Citations

Abstract

Often experimentalists require a quantitative assessment of the levels of heterologously expressed proteins to best interpret changed Ca(2+) signaling patterns. Here, we detail a rapid and convenient western blotting method for individual Xenopus oocytes. The method exploits recently introduced rapid blotting systems, commercially available from Invitrogen (iBlot) or Bio-Rad (Trans-Blot Turbo). The key advantage is speed: from live cell to transferred membrane in <1 h. Therefore, oocytes can be conveniently processed for western blotting to assess relative expression levels, even after a long day of Ca(2+) imaging experiments.

Author List

Lin-Moshier Y, Marchant JS

Author

Jonathan S. Marchant PhD Chair, Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Blotting, Western
Molecular Biology
Oocytes
Recombinant Proteins
Xenopus