Faculty Collaboration Database

Role of adenosine receptors in late preconditioning against myocardial stunning in conscious rabbits. Am J Physiol 1997 Sep;273(3 Pt 2):H1324-32

Date

10/10/1997

Pubmed ID

9321822

DOI

10.1152/ajpheart.1997.273.3.H1324

Scopus ID

2-s2.0-33751268691 (requires institutional sign-in at Scopus site)   53 Citations

Abstract

Conscious rabbits underwent six 4-min coronary occlusions interspersed with 4-min periods of reperfusion for 2 consecutive days (days 1 and 2 of stage I); 2 wk later, they underwent the same protocol (days 1 and 2 of stage II) except that they received either 8-(p-sulfophenyl)theophylline (SPT) on day 1 (group I, n = 5) or 2-chloro-N6-cyclopentyl-adenosine (CCPA) on the day before day 1 (group II, n = 6). In both groups I and II, on day 1 of stage I, systolic wall thickening (WTh) remained significantly depressed for several hours, indicating myocardial stunning; on day 2, however, the total deficit of WTh was approximately 50% less than on day 1 (P < 0.01), indicating the development of late preconditioning (PC) against myocardial stunning. Despite administration of SPT, in group I the deficit of WTh during stage II was 55% less on day 2 than on day 1 (P < 0.05). Similar results were obtained in three other rabbits treated with PD-115199 on day 1. In group II, pretreatment with CCPA during stage II failed to decrease the deficit of WTh on day 1. This study presents a new conscious rabbit model for studying myocardial stunning that is relatively inexpensive and technically less demanding than larger animal models. In this model, the development of late PC against myocardial stunning is not blocked by nonselective blockade of adenosine receptors with either SPT or PD-115199, nor is it induced by activation of adenosine A1 receptors with CCPA, indicating that adenosine receptors are not involved in the pathogenesis of this phenomenon.

Author List

Maldonado C, Qiu Y, Tang XL, Cohen MV, Auchampach J, Bolli R

Author

John A. Auchampach PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Blood Pressure
Electrocardiography
Heart Rate
Hemodynamics
Ischemic Preconditioning, Myocardial
Male
Myocardial Reperfusion
Myocardial Stunning
Purinergic P1 Receptor Antagonists
Purines
Rabbits
Receptors, Purinergic P1
Sulfonamides
Systole
Theophylline
Time Factors
Ventricular Function, Left