Faculty Collaboration Database

Differential osteopontin expression in human osteoblasts derived from iliac crest and alveolar bone and its role in early stages of angiogenesis. J Bone Miner Metab 2019 Jan;37(1):105-117

Date

01/13/2018

Pubmed ID

29327303

DOI

10.1007/s00774-017-0900-1

Scopus ID

2-s2.0-85040341380 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

In our previous study, we revealed significant differences of osteopontin (OPN) gene expression in primary human osteoblasts (HOBs) derived from iliac crest bone (iHOBs) and alveolar bone (aHOBs). The present study aims at assigning this discriminative expression to a possible biologic function. OPN is known to be involved in several pathologic and physiologic processes, among others angiogenesis. Therefore, we studied the reaction of human umbilical vein endothelial cells (HUVECs) to HOB-derived OPN regarding angiogenesis. To this end, human primary explant cultures of both bone entities from ten donors were established. Subsequent transcription analysis detected higher gene expression of OPN in iHOBs compared to aHOBs, thereby confirming the results of our previous study. This difference was particularly apparent when cultures were derived from female donors. Hence, OPN protein expression as well as the angiogenic potential of OPN was analyzed, originating from HOBs of one female donor. In accordance to the gene expression level, secreted OPN was more abundant in the supernatant of iHOBs than in aHOBs. Moreover, secreted OPN was found to stimulate migration of HUVECs, but not proliferation or tube formation. These results indicate an involvement in very early stages of angiogenesis and a functional distinction of OPN from HOBs derived from different bone entities.

Author List

Wein M, Huelter-Hassler D, Nelson K, Fretwurst T, Nahles S, Finkenzeller G, Altmann B, Steinberg T

Author

Melissa Wein MD Assistant Professor in the Radiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Alveolar Process
Animals
Cell Movement
Cell Proliferation
Female
Gene Expression Regulation
Human Umbilical Vein Endothelial Cells
Humans
Ilium
Neovascularization, Physiologic
Osteoblasts
Osteopontin
RNA, Messenger