Faculty Collaboration Database

Expression analysis of inflammasome sensors and implication of NLRP12 inflammasome in prostate cancer. Sci Rep 2017 Jun 29;7(1):4378

Date

07/01/2017

Pubmed ID

28663562

Pubmed Central ID

PMC5491527

DOI

10.1038/s41598-017-04286-4

Scopus ID

2-s2.0-85021638100 (requires institutional sign-in at Scopus site)   42 Citations

Abstract

Inflammasomes are multi-proteins complex regulating inflammation-associated signaling. While inflammation plays a critical role in cancer cell growth, studies remain uncharacterized on the role of inflammasomes in prostate cancer. Using Gene Expression Omnibus (GEO) public datasets, we screened the expression profiles of inflammasome sensors NLRP3, NLRC4, NLRP6, NRLP12, and AIM2 in prostate tumor tissues, and verified their mRNA level in a panel of prostate cancer cell lines. The selected expression of NLRP3 and NLRP12 inflammasomes was validated, and the clinical association was evaluated in human prostate archival tumor tissues. We observed that the expression of inflammasome sensors was dysregulated at the mRNA level except for the NLRP12. The intensity of NLRP12 immunostaining was significantly higher in malignant prostate as compared to their adjacent benign tissues. In contrast, the NLRP3 immunostaining in prostate tissues was heterogeneous. The inflammasome complex proteins ASC (apoptosis-associated speck-like protein containing a CARD) and pro-caspase-1, as well as its downstream targets IL-1β and IL-18 were confined to aggressive prostate cancer cells. These data suggest an increased expression of NLRP12 in association with prostate cancer and support the role of NLRP12 inflammasome complex regulating inflammatory cytokines in understanding the role of inflammation in the prostate cancer.

Author List

Karan D, Tawfik O, Dubey S

Author

Dev Karan PhD Associate Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Cell Line, Tumor
Computational Biology
Databases, Genetic
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Inflammasomes
Intracellular Signaling Peptides and Proteins
Male
NLR Family, Pyrin Domain-Containing 3 Protein
Prostatic Neoplasms