Dysregulated expression of MIC-1/PDF in human prostate tumor cells. Biochem Biophys Res Commun 2003 Jun 06;305(3):598-604
Date
05/24/2003Pubmed ID
12763036DOI
10.1016/s0006-291x(03)00823-4Scopus ID
2-s2.0-0038578603 (requires institutional sign-in at Scopus site) 60 CitationsAbstract
As a part of the study to identify genes associated with hormone-refractory stage of human prostate cancer, we have recently identified several genetic and epigenetic changes that seem to be associated with the progression of androgen-sensitive to androgen-independent prostate tumor cells. In the present study, we report a novel gene, macrophage inhibitory cytokine-1 (MIC-1) also known as prostate derived factor (PDF), that was highly expressed in androgen-independent LNCaP-C81 cells and its metastatic variant LNCaP-Ln3 compared to androgen-sensitive LNCaP-C33 cells. The MIC-1/PDF expression was dysregulated (very low to non-detectable) in the androgen-independent PC3 and DU145 cells. Interestingly, serum factors demonstrated a differential regulation of MIC-1/PDF in the androgen-sensitive and the androgen-independent cells of LNCaP cells. Immunohistochemical analysis on 15 prostatic adenocarcinomas showed a weak staining in the benign prostatic glandular area (intensity score 2.38+/-0.25; n=13), while the immunoreactivity was significantly stronger (p<0.05) in areas of adenocarcinoma (score 7.33+/-0.88; n=15). Altogether, these data suggest that the serum factors (including androgens and cytokines) might contribute to the regulation of the MIC-1/PDF gene that seems to be associated with the progression of prostate cancer.
Author List
Karan D, Chen SJ, Johansson SL, Singh AP, Paralkar VM, Lin MF, Batra SKAuthor
Dev Karan PhD Associate Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdenocarcinomaBone Morphogenetic Proteins
Cell Culture Techniques
Cytokines
Disease Progression
Gene Expression Regulation, Neoplastic
Growth Differentiation Factor 15
Humans
Immunohistochemistry
Male
Membrane Proteins
Prostatic Neoplasms
RNA, Messenger
Tumor Cells, Cultured
