Faculty Collaboration Database

Hydroxyethyl methacrylate grafted carboxy methyl tamarind (CMT-g-HEMA) polysaccharide based matrix as a suitable scaffold for skin tissue engineering. Carbohydr Polym 2018 Jun 01;189:87-98

Date

03/28/2018

Pubmed ID

29580430

DOI

10.1016/j.carbpol.2018.01.079

Scopus ID

2-s2.0-85041818037 (requires institutional sign-in at Scopus site)   21 Citations

Abstract

Patho-physiologies related to skin are diverse in nature such as burns, skin ulcers, atopic dermatitis, psoriasis etc. which impose severe bio-medical problems and thus enforce requirement of new and healthy skin prepared through tissues engineering methodologies. However, fully functional and biodegradable matrix for attachment, growth, proliferation and differentiation of the relevant cells is not available. In the present study, we introduce a set of hydrogels synthesized by incorporation of a synthetic monomer (Hydroxyethlmethacryate) with a semi-synthetic polymer backbone (carboxy methyl tamarind, CMT) in different mole ratios. We termed these materials as CMT:HEMA based hydrogels and these were characterized by different physico-chemical techniques, namely by X-Ray Diffraction, SEM and Dynamic Light Scattering. Biocompatibility studies with HaCaT, NIH-3T3 and mouse dermal fibroblasts confirm that this material is biocompatible. MTT assay further confirmed that this material does not have any cytotoxic effects. Assays for mitochondrial functionality such as ATP assay and mitochondrial reactive oxygen (ROS) generation also suggest that this material is safe and does not have any cytotoxicity. Hemolytic assay with red blood cells and acute skin irritation test on SD Rats confirmed that this material is suitable for ex-vivo application in future. We suggest that this hydrogel is suitable for in-vivo applications and may have clinical and commercial importance against skin disorders.

Author List

Choudhury P, Kumar S, Singh A, Kumar A, Kaur N, Sanyasi S, Chawla S, Goswami C, Goswami L

Author

Priyanka Choudhury PhD Postdoctoral Fellow in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cell Line, Tumor
Cells, Cultured
Female
Humans
Male
Methacrylates
Polysaccharides
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species
Tissue Engineering
Tissue Scaffolds
X-Ray Diffraction