Faculty Collaboration Database

Stability of global methylation profiles of whole blood and extracted DNA under different storage durations and conditions. Epigenomics 2018 Jun;10(6):797-811

Date

04/24/2018

Pubmed ID

29683333

DOI

10.2217/epi-2018-0025

Scopus ID

2-s2.0-85049644277 (requires institutional sign-in at Scopus site)   42 Citations

Abstract

AIM: To test whether DNA samples stored for a prolonged period (20 years) under various storage conditions could be used for comparative methylation studies using reduced representation bisulfite sequencing.

PATIENTS & METHODS: Five groups of human blood DNA samples (n = 5-6/group) were compared. The groupings were based on the anticoagulant used and storage temperature and duration.

RESULTS: Methylation profiles of defined genomic regions in the DNA or blood samples archived for 20 years were similar across all storage temperatures, including 4°C. The level of intersample similarity in archived samples was not significantly different than that in recently collected samples.

CONCLUSION: Archived samples, including DNA stored at 4°C for 20 years, are suitable for comparative studies of DNA methylation.

Author List

Li Y, Pan X, Roberts ML, Liu P, Kotchen TA, Cowley AW Jr, Mattson DL, Liu Y, Liang M, Kidambi S

Authors

Allen W. Cowley Jr PhD Professor in the Physiology department at Medical College of Wisconsin
Srividya Kidambi MD Sr Medical Director, Chief, Professor in the Medicine department at Medical College of Wisconsin
Michelle L. Roberts Postdoctoral Researcher 3 in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

DNA
DNA Methylation
Humans
Specimen Handling
Temperature