Faculty Collaboration Database

Variation in nuclear size and PD-L2 positivity correlate with aggressive chromophobe renal cell carcinoma. Ann Diagn Pathol 2018 Jun;34:31-35

Date

04/18/2018

Pubmed ID

29661724

DOI

10.1016/j.anndiagpath.2018.01.002

Scopus ID

2-s2.0-85040239426 (requires institutional sign-in at Scopus site)   1 Citation

Abstract

Chromophobe renal cell carcinoma (CRCC) is not amenable to International Society for Urologic Pathology-endorsed nucleolar grading. Novel grading approaches were proposed, but the rarity of adverse pathology hampers their discriminatory value. We investigate simple linear micrometer measurements and a proposed immunostain in CRCCs. 32 patients' CRCCs were studied: 12 adverse cases (stage pT3, recurrence, or metastasis), 15 controls (stage ≤pT2, no recurrence or metastasis after >3 years), and 8 metastases (3 were paired with primary adverse cases). The ratio of greatest dimensions of largest and smallest nuclei, in each of 5 "worst" high-power fields, excluding those with degenerative features, was designated variation in nuclear size (VNS). Percent multinucleate cells (PMC) were also counted. Mouse anti PD-L2 monoclonal antibody immunostaining was performed. Mean VNS measured in adverse primary and control primary tumors were 3.7 ± 0.5 and 2.4 ± 0.4 respectively (P < .001), and 3.4 ± 0.4 for metastases (P < .001). Optimal VNS cut-off was 2.5, with sensitivity and specificity 0.85 and 0.81, respectively. PMCs were 6.0 ± 3.0 for adverse group, 5.7 ± 2.7 for controls, and 4.1 ± 1.6 for metastases (P = NS). PD-L2 could not discriminate adverse versus good primary tumors (χ²1.6, P = .2), but was higher in metastases (χ² 6.9, P < .01), or metastases plus adverse primary tumors (χ² 4.8, P = .03), compared to good-pathology primary tumors. In conclusion, VNS is an easily obtained measurement that can predict adverse behavior of chromophobe RCC, and may impart value for needle biopsy reporting and the choice of active surveillance. PD-L2 was elevated in metastases but was less useful for primary tumors.

Author List

Mostafa ME, Abdelkader A, Kuroda N, Pérez-Montiel D, Banerjee A, Hes O, Iczkowski KA

Author

Anjishnu Banerjee PhD Associate Professor in the Institute for Health and Equity department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Aged
Aged, 80 and over
Animals
Antibodies, Monoclonal
Carcinoma, Renal Cell
Cell Nucleus
Cell Nucleus Size
Female
Humans
Kidney Neoplasms
Male
Mice
Middle Aged
Prognosis
Programmed Cell Death 1 Ligand 2 Protein
Young Adult