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Pretransplant cytotoxic conditioning produces effects consistent with clonal deletion mechanisms. J Surg Res 1987 May;42(5):498-502

Date

05/01/1987

Pubmed ID

3295388

DOI

10.1016/0022-4804(87)90024-2

Scopus ID

2-s2.0-0023186465   1 Citation

Abstract

A rat cardiac allograft model (ACl to Lewis) was used to investigate the clonal deletion theory. Twelve groups of Lewis recipients received various combinations of donor-specific blood transfusions (DSTs), immediate post-DST immunosuppression with azathioprine/prednisone, and low-dose cyclosporine (1 mg/kg/day) posttransplant. DSTs and cyclosporine together gave modest prolongation of graft survival (from 6.0 to 17 days). DSTs plus immediate post-DST immunosuppression followed by low-dose cyclosporine prolonged graft survival to an average of 45 days. Third-party transfusions alone and in combination with immunosuppression did not significantly prolong allograft survival. Postoperative cyclosporine was required for the expression of this effect suggesting that clonal depression rather than clonal deletion had occurred. Combining DSTs with brief but intense preoperative immunosuppression may be a more effective method of pretransplant conditioning than DSTs alone.

Author List

Zheng TL, Johnson CP, Sutherland DE

Author

Christopher P. Johnson MD Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Azathioprine
Blood Transfusion
Clone Cells
Cyclosporins
Graft Survival
Heart Transplantation
Immunosuppressive Agents
Male
Postoperative Care
Prednisone
Preoperative Care
Rats
Rats, Inbred Strains
jenkins-FCD Prod-478 d1509cf07a111124a2d122fd3df854cc0b993c00