Pretransplant cytotoxic conditioning produces effects consistent with clonal deletion mechanisms. J Surg Res 1987 May;42(5):498-502
Date
05/01/1987Pubmed ID
3295388DOI
10.1016/0022-4804(87)90024-2Scopus ID
2-s2.0-0023186465 (requires institutional sign-in at Scopus site) 1 CitationAbstract
A rat cardiac allograft model (ACl to Lewis) was used to investigate the clonal deletion theory. Twelve groups of Lewis recipients received various combinations of donor-specific blood transfusions (DSTs), immediate post-DST immunosuppression with azathioprine/prednisone, and low-dose cyclosporine (1 mg/kg/day) posttransplant. DSTs and cyclosporine together gave modest prolongation of graft survival (from 6.0 to 17 days). DSTs plus immediate post-DST immunosuppression followed by low-dose cyclosporine prolonged graft survival to an average of 45 days. Third-party transfusions alone and in combination with immunosuppression did not significantly prolong allograft survival. Postoperative cyclosporine was required for the expression of this effect suggesting that clonal depression rather than clonal deletion had occurred. Combining DSTs with brief but intense preoperative immunosuppression may be a more effective method of pretransplant conditioning than DSTs alone.
Author List
Zheng TL, Johnson CP, Sutherland DEAuthor
Christopher P. Johnson MD Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAzathioprine
Blood Transfusion
Clone Cells
Cyclosporins
Graft Survival
Heart Transplantation
Immunosuppressive Agents
Male
Postoperative Care
Prednisone
Preoperative Care
Rats
Rats, Inbred Strains
