Faculty Collaboration Database

Neutrophils in chronic and aggressive periodontitis in interaction with Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. J Periodontal Res 2009 Jun;44(3):368-77

Date

02/13/2009

Pubmed ID

19210340

Pubmed Central ID

PMC4180098

DOI

10.1111/j.1600-0765.2008.01113.x

Scopus ID

2-s2.0-65349143676 (requires institutional sign-in at Scopus site)   66 Citations

Abstract

BACKGROUND AND OBJECTIVE: This study analyzed the interaction of Porphyromonas gingivalis ATCC 33277 and Aggregatibacter actinomycetemcomitans Y4 with peripheral blood polymorphonuclear neutrophils taken from patients with aggressive periodontitis and chronic periodontitis.

MATERIAL AND METHODS: Peripheral blood polymorphonuclear neutrophils obtained from 12 patients with chronic periodontitis, six patients with aggressive periodontitis and 12 healthy controls were exposed to P. gingivalis and A. actinomycetemcomitans following opsonization of the bacteria using the patient's own serum. Serum immunoglobulin G (IgG) levels against both periodontopathogens were measured. Phagocytosis and killing of the bacteria, as well as the extracellular human neutrophil elastase activity, were quantified. The total amount and the extracellular release of reactive oxygen species were measured using luminol-dependent and isoluminol-dependent chemiluminescence.

RESULTS: Polymorphonuclear neutrophils from patients with chronic (62.16 +/- 19.39%) and aggressive (43.26 +/- 26.63%) periodontitis phagocytosed more P. gingivalis than the healthy controls (24.43 +/- 19.87%) at the 30-min time point after exposure to the bacteria (p < 0.05). High serum IgG levels against P. gingivalis and A. actinomycetemcomitans were detected in subjects with periodontitis. Polymorphonuclear neutrophils from subjects with chronic and aggressive periodontitis released significantly more reactive oxygen species and demonstrated greater human neutrophil elastase activity in the absence of any stimulus than polymorphonuclear neutrophils from healthy controls (p < 0.05). Polymorphonuclear neutrophils in chronic periodontitis released significantly more reactive oxygen species when exposed to P. gingivalis and A. actinomycetemcomitans than polymorphonuclear neutrophils in aggressive periodontitis.

CONCLUSION: High serum IgG levels against P. gingivalis and A. actinomycetemcomitans promote phagocytosis in periodontitis. The extracellular release of reactive oxygen species and neutrophil elastase by polymorphonuclear neutrophils may also contribute to damage of the surrounding periodontal tissues.

Author List

Guentsch A, Puklo M, Preshaw PM, Glockmann E, Pfister W, Potempa J, Eick S

Author

Arndt Geuntsch in the CTSI department at Medical College of Wisconsin - CTSI

MESH terms used to index this publication - Major topics in bold

Adult
Aggregatibacter actinomycetemcomitans
Aggressive Periodontitis
Antibodies, Bacterial
Case-Control Studies
Chronic Periodontitis
Female
Humans
Immunoglobulin G
Leukocyte Elastase
Luminescence
Male
Middle Aged
Neutrophils
Phagocytosis
Porphyromonas gingivalis
Reactive Oxygen Species