Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities. Mol Biol Cell 2016 Jul 01;27(13):1990-9
Date
05/06/2016Pubmed ID
27146114Pubmed Central ID
PMC4927273DOI
10.1091/mbc.E15-12-0848Scopus ID
2-s2.0-84976601365 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
Microtubule (MT)-binding centromere protein F (CENP-F) was previously shown to play a role exclusively in chromosome segregation during cellular division. Many cell models of CENP-F depletion show a lag in the cell cycle and aneuploidy. Here, using our novel genetic deletion model, we show that CENP-F also regulates a broader range of cellular functions outside of cell division. We characterized CENP-F(+/+) and CENP-F(-/-) mouse embryonic fibroblasts (MEFs) and found drastic differences in multiple cellular functions during interphase, including cell migration, focal adhesion dynamics, and primary cilia formation. We discovered that CENP-F(-/-) MEFs have severely diminished MT dynamics, which underlies the phenotypes we describe. These data, combined with recent biochemical research demonstrating the strong binding of CENP-F to the MT network, support the conclusion that CENP-F is a powerful regulator of MT dynamics during interphase and affects heterogeneous cell functions.
Author List
Pfaltzgraff ER, Roth GM, Miller PM, Gintzig AG, Ohi R, Bader DMAuthor
Gretchen M. Roth MD Associate Professor in the Dermatology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Cycle
Centromere
Chromosomal Proteins, Non-Histone
Chromosome Aberrations
Chromosome Segregation
Fibroblasts
Interphase
Kinetochores
Mice
Mice, Knockout
Microfilament Proteins
Microtubules
Mitosis
Protein Binding
