Protection by dietary proteins against the effects of bile acids on rat jejunum and stomach. Gastroenterology 1985 May;88(5 Pt 1):1183-91
Date
05/01/1985Pubmed ID
2579867DOI
10.1016/s0016-5085(85)80078-0Scopus ID
2-s2.0-0021986948 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
Because bile acids bind to certain proteins we examined whether the effect of dihydroxy bile acids on jejunal water transport and gastric mucosal function could be blocked by the presence of protein. In the rat jejunum 2.5% bovine serum albumin blocked the secretion of water and electrolytes induced by 2 mM deoxycholate, whereas 5% ovalbumin, which does not bind bile acids, had no effect. Bovine serum albumin protected large unilamellar liposomes from damage by taurodeoxycholate and reduced the monomer concentration of taurodeoxycholate, whereas ovalbumin afforded no protection. In equilibrium dialysis studies whey protein and bovine serum albumin reduced the free taurodeoxycholate concentration (150 mM HCl enhanced this effect). In the rat stomach taurodeoxycholate (2.5 or 10 mM) in the presence of 150 mM HCl reduced potential difference and enhanced sodium secretion and hydrogen ion loss. These effects were reduced in the presence of whey protein. We conclude that proteins that bind bile acids have the potential to protect mucosal membranes from the adverse effects of bile acids.
Author List
Kruidenier JF, Ammon HV, Charaf UK, Walter LGAuthor
Helmut V. Ammon MD Adjunct Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBile Acids and Salts
Biological Transport, Active
Deoxycholic Acid
Dietary Proteins
Gastric Mucosa
Intestinal Absorption
Intestinal Mucosa
Ion Channels
Jejunum
Lactose
Liposomes
Male
Ovalbumin
Protein Binding
Rats
Rats, Inbred Strains
Serum Albumin, Bovine
Taurodeoxycholic Acid
