Comparison of cholesterol and beta-sitosterol: effects on jejunal fluid secretion induced by oleate, and absorption from mixed micellar solutions. Gut 1983 Jul;24(7):653-8
Date
07/01/1983Pubmed ID
6862286Pubmed Central ID
PMC1420048DOI
10.1136/gut.24.7.653Scopus ID
2-s2.0-0020626032 (requires institutional sign-in at Scopus site) 31 CitationsAbstract
Jejunal fluid secretion induced by perfusion with oleic acid can be reduced by the addition of cholesterol. The present study was performed to test the specificity of this effect by comparing the effects of cholesterol with that of a plant sterol, beta-sitosterol during perfusion of the jejunum in healthy volunteers. In addition, we compared the solubilities of cholesterol and beta-sitosterol in micellar solutions and their jejunal absorption rates. One millimolar beta-sitosterol was as effective as 1 mM cholesterol in reducing jejunal fluid secretion induced by 6 mM oleate (n = 7). In mixed micellar solutions consisting of 10 mM taurocholate and 6 mM oleate, solubility of beta-sitosterol is about one third of cholesterol solubility. When cholesterol was gradually replaced by beta-sitosterol in the incubation mixture, beta-sitosterol reduced cholesterol solubility to a greater extent than would be expected from an equimolar replacement of cholesterol by beta-sitosterol. Absorption of beta-sitosterol was limited by its solubility in mixed micellar solutions and both sterols were absorbed at equal rates as long as their solubility limits were not exceeded (n = 5).
Author List
Slota T, Kozlov NA, Ammon HVAuthor
Helmut V. Ammon MD Adjunct Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
CholesterolHumans
Intestinal Absorption
Intestinal Secretions
Jejunum
Male
Micelles
Oleic Acid
Oleic Acids
Perfusion
Sitosterols
Solubility
