Cholesterol reduces the effects of dihydroxy bile acids and fatty acids on water and solute transport in the human jejunum. J Clin Invest 1980 Apr;65(4):920-5
Date
04/01/1980Pubmed ID
7358850Pubmed Central ID
PMC434481DOI
10.1172/JCI109746Scopus ID
2-s2.0-0018901345 (requires institutional sign-in at Scopus site) 15 CitationsAbstract
Jejunal perfusion studies were performed in 16 healthy volunteers to test the hypothesis that intraluminal cholesterol can mitigate the fluid secretion induced by dihydroxy bile acids and fatty acids. Fluid secretion in the presence of 5 mM taurodeoxycholate was somewhat reduced by 4 mM mono-olein which was used for the solubilization of cholesterol. Addition of 0.8 mM cholesterol reduced fluid secretion further (P less than 0.05). Fluid secretion induced by 4 mM oleic acid was changed to net absorption in a linear fashion with increasing cholesterol concentration in the perfusion solutions. 1 mM cholesterol reduced fluid secretion induced by 6 mM oleic acid (P less than 0.005), but had no effect on fluid secretion induced by 6 mM linolenic acid. Glucose absorption was generally affected in a similar manner as water transport. In vitro, 1 mM cholesterol reduced monomer activity of 6 mM oleic acid to 72.3 +/- 0.9% of control and that of linolenic acid to 81.1 +/- 1.7% of control. Although statistically significant (P less than 0.001), the difference in the effects of cholesterol on monomer activities of the two fatty acids was rather small and it is unlikely that changes in monomer concentration of fatty acids and bile acids account for the protective effect of cholesterol. The in vivo observations point to a new physiological role for biliary cholesterol: the modification of the response of the small intestine to the effects of dihydroxy bile acids and fatty acids.
Author List
Broor SL, Slota T, Ammon HVAuthor
Helmut V. Ammon MD Adjunct Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Bile Acids and SaltsBiological Transport
Cholesterol
Fatty Acids
Glucose
Humans
Intestinal Absorption
Intestinal Mucosa
Intestines
Jejunum
Linolenic Acids
Lipid Metabolism
Male
Micelles
Oleic Acids
Water
