Faculty Collaboration Database

Vascularization is delayed in long nerve constructs compared with nerve grafts. Muscle Nerve 2016 Aug;54(2):319-21

Date

05/05/2016

Pubmed ID

27144718

Pubmed Central ID

PMC4940276

DOI

10.1002/mus.25173

Scopus ID

2-s2.0-84978800302 (requires institutional sign-in at Scopus site)   23 Citations

Abstract

INTRODUCTION: Nerve regeneration across nerve constructs, such as acellular nerve allografts (ANAs), is inferior to nerve auto/isografts especially in the case of long defect lengths. Vascularization may contribute to poor regeneration. The time course of vascular perfusion within long grafts and constructs was tracked to determine vascularization.

METHODS: Male Lewis rat sciatic nerves were transected and repaired with 6 cm isografts or ANAs. At variable days following grafting, animals were perfused with Evans Blue albumin, and grafts were evaluated for vascular perfusion by a blinded observer.

RESULTS: Vascularization at mid-graft was re-established within 3-4 days in 6 cm isografts, while it was established after 10 days in 6 cm ANAs.

CONCLUSIONS: Vascular perfusion is reestablished over a shorter time course in long isografts when compared with long ANAs. The differences in vascularization of long ANAs compared with auto/isografts suggest regenerative outcomes across ANAs could be affected by vascularization rates. Muscle Nerve 54: 319-321, 2016.

Author List

Farber SJ, Hoben GM, Hunter DA, Yan Y, Johnson PJ, Mackinnon SE, Wood MD

Author

Gwendolyn M B Hoben MD Assistant Professor in the Plastic Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Disease Models, Animal
Isografts
Male
Neovascularization, Pathologic
Nerve Regeneration
Platelet Endothelial Cell Adhesion Molecule-1
Rats
Rats, Inbred Lew
Sciatic Neuropathy
Time Factors
Transplantation, Homologous