Discovery and characterization of novel vascular and hematopoietic genes downstream of etsrp in zebrafish. PLoS One 2009;4(3):e4994
Date
03/25/2009Pubmed ID
19308258Pubmed Central ID
PMC2654924DOI
10.1371/journal.pone.0004994Scopus ID
2-s2.0-63449128252 (requires institutional sign-in at Scopus site) 41 CitationsAbstract
The transcription factor Etsrp is required for vasculogenesis and primitive myelopoiesis in zebrafish. When ectopically expressed, etsrp is sufficient to induce the expression of many vascular and myeloid genes in zebrafish. The mammalian homolog of etsrp, ER71/Etv2, is also essential for vascular and hematopoietic development. To identify genes downstream of etsrp, gain-of-function experiments were performed for etsrp in zebrafish embryos followed by transcription profile analysis by microarray. Subsequent in vivo expression studies resulted in the identification of fourteen genes with blood and/or vascular expression, six of these being completely novel. Regulation of these genes by etsrp was confirmed by ectopic induction in etsrp overexpressing embryos and decreased expression in etsrp deficient embryos. Additional functional analysis of two newly discovered genes, hapln1b and sh3gl3, demonstrates their importance in embryonic vascular development. The results described here identify a group of genes downstream of etsrp likely to be critical for vascular and/or myeloid development.
Author List
Gomez GA, Veldman MB, Zhao Y, Burgess S, Lin SAuthor
Matthew B. Veldman PhD Assistant Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBlood Vessels
Embryo, Nonmammalian
Gene Expression Profiling
Gene Expression Regulation
Hematopoiesis
Neovascularization, Physiologic
Nerve Tissue Proteins
Proteoglycans
Transcription Factors
Zebrafish
Zebrafish Proteins
