In vivo mutagenesis using EZ-Tn5. Methods Enzymol 2007;421:17-21
Date
03/14/2007Pubmed ID
17352911DOI
10.1016/S0076-6879(06)21003-6Scopus ID
2-s2.0-33847678145 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
Epicentre Biotechnologies has developed a suite of transposon-based tools for use in modern bacterial genetics. This chapter highlights the EZ-Tn5 Transposome system and focuses on in vivo mutagenesis and subsequent rescue cloning. Many other applications and variations have been described and are available through Epicentre's website at http://www.epibio.com/.
Author List
Kirby JRAuthor
John Kirby PhD Chair, Center Associate Director, Professor in the Microbiology and Immunology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
DNA Transposable ElementsDNA, Bacterial
Genome, Bacterial
Mutagenesis, Insertional
Transposases