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In vivo mutagenesis using EZ-Tn5. Methods Enzymol 2007;421:17-21

Date

03/14/2007

Pubmed ID

17352911

DOI

10.1016/S0076-6879(06)21003-6

Scopus ID

2-s2.0-33847678145   9 Citations

Abstract

Epicentre Biotechnologies has developed a suite of transposon-based tools for use in modern bacterial genetics. This chapter highlights the EZ-Tn5 Transposome system and focuses on in vivo mutagenesis and subsequent rescue cloning. Many other applications and variations have been described and are available through Epicentre's website at http://www.epibio.com/.

Author List

Kirby JR

Author

John Kirby PhD Chair, Center Associate Director, Professor in the Microbiology and Immunology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

DNA Transposable Elements
DNA, Bacterial
Genome, Bacterial
Mutagenesis, Insertional
Transposases
jenkins-FCD Prod-486 e3098984f26de787f5ecab75090d0a28e7f4f7c0