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Calcium oxalate crystal interaction with rat renal inner papillary collecting tubule cells. J Urol 1987 Sep;138(3):640-3

Date

09/01/1987

Pubmed ID

3625872

DOI

10.1016/s0022-5347(17)43289-7

Scopus ID

2-s2.0-0023281136 (requires institutional sign-in at Scopus site)   32 Citations

Abstract

Rat renal inner papillary collecting tubule cells (RPCT) have been isolated and maintained in primary culture. The cells have been found to be of only one type and they have maintained the characteristics of RPCT cells. The RPCT cells in culture appear as a monolayer with intermittent clumps of rounded cells. When small calcium oxalate monohydrate crystals (COM) or calcium oxalate dihydrate crystals (COD) are added to the monolayer of RPCT cells, the crystals bind on or about these clumps of rounded-up cells. The use of this system as a model for the study of crystal membrane interactions in crystalluria and urolithiasis is discussed.

Author List

Wiessner JH, Kleinman JG, Blumenthal SS, Garancis JC, Mandel GS, Mandel NS

Author

Samuel S. Blumenthal MD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Calcium Oxalate
Cells, Cultured
Crystallization
Kidney Calculi
Kidney Tubules
Kidney Tubules, Collecting
Male
Microscopy, Electron
Rats