Faculty Collaboration Database

Neutrophil degranulation responses to combinations of arachidonate metabolites and platelet-activating factor. Res Commun Chem Pathol Pharmacol 1984 Jan;43(1):3-23

Date

01/01/1984

Pubmed ID

6322256

Scopus ID

2-s2.0-0021325520 (requires institutional sign-in at Scopus site)   33 Citations

Abstract

Polymorphonuclear neutrophils, when stimulated, rapidly form platelet-activating factor (PAF) and metabolize their arachidonate into 5-hydroperoxyeicosatetraenoate (5-HPETE), 5-hydroxyeicosatetraenoate (5-HETE), leukotriene (LT)A4, and LTB4. PAF and LTB4 degranulate neutrophils; 5-HETE, while lacking intrinsic degranulating actions, potentiates these responses. We now find that: a) 5-HPETE similarly potentiates the two lipids and has weak degranulating actions, b) LTA4 and LTB4 degranulate neutrophils by a common pathway, c) PAF degranulates neutrophils by a distinctly different pathway, d) the actions of either LT are additive to those of PAF, e) 5-HETE is particularly effective in potentiating response to combinations of PAF and LTB4, and f) combinations of the lipids partially circumvent requirements for cytochalasin B in these degranulation responses. Thus, the five lipids can be classified into potentiators (i.e., 5-HPETE and 5-HETE) and two types of independently acting agonists (i.e., LT's are one type, PAF a second type). At low concentrations, potentiator, LT, and PAF can all interact to produce prominent responses. They may similarly interact to promote function within their cells of origin.

Author List

O'Flaherty JT, Wykle RL, Thomas MJ, McCall CE

Author

Michael J. Thomas PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Arachidonic Acids
Cytoplasmic Granules
Humans
Hydroxyeicosatetraenoic Acids
Leukotriene A4
Leukotriene B4
Leukotrienes
Neutrophils
Platelet Activating Factor
Stimulation, Chemical