Identification and quantitation of electron-transport components in human polymorphonuclear neutrophils. Biochim Biophys Acta 1982 Dec 15;682(3):430-5
Date
12/15/1982Pubmed ID
6295472DOI
10.1016/0005-2728(82)90057-3Scopus ID
2-s2.0-0020460996 (requires institutional sign-in at Scopus site) 21 CitationsAbstract
Using dithionite difference spectra we have detected cytochrome b in highly purified human neutrophils at a concentration of 0.08 nmol/mg protein. The presence of quinone was identified in lipid extracts at a concentration of approx. 0.06 nmol/mg protein. It was identified as ubiquinone-10 by mass spectrographic analysis. Simultaneous measurements of cytochrome oxidase indicated that these compounds could not be attributed to mitochondrial contamination. These results are compatible with the hypothesis that initiation of the respiratory burst in human neutrophils involves a multicomponent electron-transport system.
Author List
Cunningham CC, DeChatelet LR, Spach PI, Parce JW, Thomas MJ, Lees CJ, Shirley PSAuthor
Michael J. Thomas PhD Professor in the Pharmacology and Toxicology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Cytochrome b GroupDithionite
Electron Transport
Electron Transport Complex IV
Humans
Kinetics
Mass Spectrometry
Neutrophils