Medical College of Wisconsin
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Identification and quantitation of electron-transport components in human polymorphonuclear neutrophils. Biochim Biophys Acta 1982 Dec 15;682(3):430-5

Date

12/15/1982

Pubmed ID

6295472

DOI

10.1016/0005-2728(82)90057-3

Scopus ID

2-s2.0-0020460996 (requires institutional sign-in at Scopus site)   21 Citations

Abstract

Using dithionite difference spectra we have detected cytochrome b in highly purified human neutrophils at a concentration of 0.08 nmol/mg protein. The presence of quinone was identified in lipid extracts at a concentration of approx. 0.06 nmol/mg protein. It was identified as ubiquinone-10 by mass spectrographic analysis. Simultaneous measurements of cytochrome oxidase indicated that these compounds could not be attributed to mitochondrial contamination. These results are compatible with the hypothesis that initiation of the respiratory burst in human neutrophils involves a multicomponent electron-transport system.

Author List

Cunningham CC, DeChatelet LR, Spach PI, Parce JW, Thomas MJ, Lees CJ, Shirley PS

Author

Michael J. Thomas PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Cytochrome b Group
Dithionite
Electron Transport
Electron Transport Complex IV
Humans
Kinetics
Mass Spectrometry
Neutrophils