The role of superoxide in xanthine oxidase-induced autooxidation of linoleic acid. J Biol Chem 1982 Jul 25;257(14):8343-7
Date
07/25/1982Pubmed ID
6282880Scopus ID
2-s2.0-0020377461 (requires institutional sign-in at Scopus site) 54 CitationsAbstract
The effect of hydroxyperoxyoctadecadienoic acid, e.g. 13-hydroperoxy-cis,9,trans-11-octadecadienoic acid, on the autooxidation of linoleic acid induced by superoxide radical was examined in a system containing xanthine oxidase, acetaldehyde, and diethylenetriaminepentaacetic acid dissolved in an aqueous phosphate buffer containing 10% ethanol. The superoxide radical is required for autooxidation, as shown by essentially complete inhibition on the addition of superoxide dismutase. Pure linoleic acid was not readily oxidized, but the addition of lipid hydroperoxide markedly stimulated the autooxidation. Addition of 2.8 microM FeCl3 did not produce an increase in the rate of xanthine oxidase-induced autooxidation. Spontaneous autooxidation, a process slower than xanthine oxidase-induced autooxidation, was detectable on the time scale of these observations but was slower than the xanthine oxidase-induced autooxidation. Initiation of linoleic acid autooxidation is postulated to result from a reaction between superoxide and lipid hydroperoxide. The nature of this reaction is uncertain, but it does not appear to depend on iron catalysis.
Author List
Thomas MJ, Mehl KS, Pryor WAAuthor
Michael J. Thomas PhD Professor in the Pharmacology and Toxicology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCatalase
Cattle
Chromatography, High Pressure Liquid
Kinetics
Linoleic Acid
Linoleic Acids
Liver
Oxidation-Reduction
Oxygen
Superoxide Dismutase
Superoxides
Xanthine Oxidase
