The effect of free radical derived hydroxyeicosatetraenoic acids on hexose transport in the human polymorphonuclear leukocyte. Prostaglandins 1982 Feb;23(2):265-72
Date
02/01/1982Pubmed ID
7079522DOI
10.1016/0090-6980(82)90054-5Scopus ID
2-s2.0-0020471108 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
The following racemic hydroxyicosatetraenoic acids were prepared and assayed for their ability to stimulate hexose transport in human polymorphonuclear leukocytes: 15-, 12-, 11-, 9-, 8-, and 5-hydroxyicosatetraenoic acids. The compounds were isolated from reduced, autoxidized arachidonic acid. The results demonstrate that only the 12- and 5-hydroxyicosatetraenoic acids are biologically active inducing half-maximal responses at 820 and 176 nM, respectively. Thus, the bioactions of hydroxicosatetraenoates ae crucially dependent upon the position of the hydroxy residue. Response to both hydroxyicosatetraenoates was effectively blocked by two inhibitors of arachidonic acid metabolism: nordihydroguaiaretic acid and indomethacin. A third arachidonic acid antimetabolite, 5, 8, 11, 14-eicosatetraynoic acid, completely inhibited the response to 12-HETE but caused only partial inhibition of the response to 5-HETE.
Author List
Thomas MJ, O'Flaherty JT, Cousart S, McCall CEAuthor
Michael J. Thomas PhD Professor in the Pharmacology and Toxicology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
12-Hydroxy-5,8,10,14-eicosatetraenoic AcidArachidonic Acids
Biological Transport, Active
Deoxy Sugars
Deoxyglucose
Free Radicals
Humans
Hydroxyeicosatetraenoic Acids
Neutrophils
