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Neutrophil-degranulating action of 5,12-dihydroxy-6,8,10,14-eicosatetraenoic acid and 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine. Comparison with other degranulating agents. Am J Pathol 1981 Dec;105(3):264-9

Date

12/01/1981

Pubmed ID

6274199

Pubmed Central ID

PMC1903896

Scopus ID

2-s2.0-0019819381 (requires institutional sign-in at Scopus site)   50 Citations

Abstract

5,12-Dihydroxy-6,8,10,14-eicosatetraenoic acid was prepared from rabbit neutrophils challenged with ionophore A23187 plus arachidonic acid. It was purified by reverse-phase high-performance liquid chromatography with the use of a two-step procedure that effectively resolved the lipid from closely eluting contaminants. The phospholipid 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine was prepared from beef heart plasmalogen. Confirming earlier reports, both lipids stimulated human polymorphonuclear neutrophils to release granule-bound enzymes. Their respective degranulating actions took less than 5 minutes to become maximal, were dependent upon the presence of cytochalasin B, and required intact pathways of arachidonic acid metabolism, as judged by the inhibitory actions of arachidonate antimetabolites. Neither lipid required more than trace concentrations of extracellular calcium to effect optimal amounts of enzyme release. In one or more of these regards the two lipids differed strikingly from other neutrophil-degranulating agents such as A23187, N-formyl-methionyl-leucyl-phenylalanine, and phorbol myristate acetate. The mechanism of action of the two lipid stimuli, therefore, appear to differ somewhat from the mechanisms of the other three stimuli.

Author List

O'Flaherty JT, Wykle RL, Lees CJ, Shewmake T, McCall CE, Thomas MJ

Author

Michael J. Thomas PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Arachidonic Acid
Arachidonic Acids
Calcimycin
Calcium
Cattle
Chemotactic Factors
Cytochalasin B
Cytoplasmic Granules
Humans
In Vitro Techniques
Leukotriene B4
Lysophosphatidylcholines
N-Formylmethionine
N-Formylmethionine Leucyl-Phenylalanine
Neutrophils
Oligopeptides
Platelet Activating Factor
Rabbits
Stimulation, Chemical
Tetradecanoylphorbol Acetate