Faculty Collaboration Database

Dopamine-induced sulfatase and its regulator are required for Salmonella enterica serovar Typhimurium pathogenesis. Microbiology (Reading) 2019 Mar;165(3):302-310

Date

01/17/2019

Pubmed ID

30648943

DOI

10.1099/mic.0.000769

Scopus ID

2-s2.0-85063199979 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

Catecholamine hormones enhance the virulence of pathogenic bacteria. Studies in the 1980s made intriguing observations that catecholamines were required for induction of sulfatase activity in many enteric pathogens, including Salmonella enterica serovar Typhimurium. In this report, we show that STM3122 and STM3124, part of horizontally acquired Salmonella pathogenesis island 13, encode a catecholamine-induced sulfatase and its regulator, respectively. Induction of sulfatase activity was independent of the well-studied QseBC and QseEF two-component regulatory systems. S. Typhimurium 14028S mutants lacking STM3122 or STM3124 showed reduced virulence in zebrafish. Because catecholamines are inactivated by sulfation in the mammalian gut, S. Typhimurium could utilize CA-induced sulfatase to access free catecholamines for growth and virulence.

Author List

Das S, Sreevidya VS, Udvadia AJ, Gyaneshwar P

Author

Ava Udvadia BS,PhD Associate Professor in the Biological Sciences department at University of Wisconsin - Milwaukee

MESH terms used to index this publication - Major topics in bold

Animals
Bacterial Proteins
Dopamine
Gene Expression Regulation, Bacterial
Genomic Islands
Microbial Viability
Mutation
Periplasm
Salmonella Infections, Animal
Salmonella typhimurium
Sulfatases
Transcription Factors
Virulence
Zebrafish