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Mesh term Heteroduplex Analysis

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Nucleic Acid Hybridization

Description

A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.



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jenkins-FCD Prod-480 9a4deaf152b0b06dd18151814fff2e18f6c05280