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Mesh term Chromatin Immunoprecipitation

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Genetic Techniques
Immunoprecipitation

Description

A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.



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jenkins-FCD Prod-468 69a93cef3257f26b866d455c1d2b2d0f28382f14