Release of pyrophosphate by normal mammalian articular hyaline and fibrocartilage in organ culture. Arthritis Rheum 1981 Dec;24(12):1522-7
Date
12/01/1981Pubmed ID
6275863DOI
10.1002/art.1780241211Scopus ID
2-s2.0-0019790796 (requires institutional sign-in at Scopus site) 56 CitationsAbstract
Calcium pyrophosphate dihydrate crystals are found most frequently in fibrocartilaginous tissue and to a lesser extent in hyaline articular cartilage. Previous investigators found that pyrophosphate (PPi) was released into the medium by immature rabbit hyaline cartilage and osteoarthritic human cartilage in organ culture but not by normal human or mature rabbit cartilage. By employing a sensitive fluorometric assay for PPi and correcting for hydrolysis of PPi during the incubations, we detected PPi release by all normal mammalian cartilage studied. PPi release per mg wet weight of lapine and canine cartilage was paralleled by uronic acid production. Meniscal fibrocartilage, the most common site of calcium pyrophosphate deposits, also elaborate PPi.
Author List
Ryan LM, Cheung HS, McCarty DJAuthor
Lawrence M. Ryan MD Emeritus Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCartilage, Articular
Diphosphates
Dogs
Humans
Macaca nemestrina
Mammals
Organ Culture Techniques
Rabbits
Uronic Acids
